Purification of the constituent enzyme fractions of mycobacillin synthetase.
نویسندگان
چکیده
The final purification of the three-fraction enzyme complex mycobacillin synthetase was done by hydroxyapatite column chromatography and sucrose-density-gradient centrifugation; each of the fractions obtained migrates as a single component in SDS/polyacrylamide-gel electrophoresis and gel electrofocusing. The Mr of the enzyme fractions A, B and C by gel filtration is 260 000, 190 000 and 105 000, and that by SDS/polyacrylamide-gel electrophoresis is 252 000, 198 000 and 108 000 respectively. None of the enzyme fractions appears to possess subunit structure.
منابع مشابه
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 235 1 شماره
صفحات -
تاریخ انتشار 1986